Agelenids of the World

Systematics and Taxonomy of Agelenidae, a Worldwide distributed Spider Family

Apoptosis and biochemical biomarkers of stress in spiders from industrially polluted areas exposed to high temperature and dimethoate

Publication Type:Journal Article
Year of Publication:2005
Authors:G. Wilczek
Journal:Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology
Date Published:2005
ISBN Number:1532-0456
Keywords:[Temperature / High temperature / ] [Poland / Olkusz & Pilica /, Abiotic factors, Agelena labyrinthica, Agelena labyrinthica (Araneae)., Apoptosis / Midgut gland, Apoptosis & biochemical biomarkers of stress, Biochemistry, biomarkers, Chemical factors, Chemical pollution, Digestive system, enzyme activity / ] [Digestive gland / / Apoptosis & chemical stress, Eurasia, Europe, gland apoptosis & biochemical stress marker responses to temperature &, individuals from metal polluted sites] [Enzymes / Antioxidant, individuals from polluted sites] [Metal pollution / / Midgut, Land zones, Life cycle and development, metallothionein / Stress biomarkers, Midgut gland apoptosis & biochemical stress marker responses], Palaearctic region, Pardosa lugubris (Araneae)., Pardosa lugubris [Proteins / Heat shock proteins &, pesticide, pesticide exposure]., pesticide relationship] [Fertilizers and pesticides / Dimethoate /, Physical factors, Pollution, Proteins, responses to high temperature &, responses to temperature &, stress biomarker response to temperature &, temperature & pesticide pollution exposure] [Development /

The aim of this study was to evaluate the relations between apoptosis and the activity of antioxidant enzymes (superoxide dismutase; catalase) and quantitative changes in stress protein positive cells (Hsp70; metallothionein) in midgut glands of funnel web spiders Agelena labyrinthica (Agelenidae) and wolf spiders Pardosa lugubris (Lycosidae) exposed to high temperature and pesticide under laboratory conditions. The spiders were collected from two meadow ecosystems differently polluted with metals (Olkusz and Pilica, southern Poland). Under stress conditions, P. lugubris had fewer apoptotic cells in the midgut glands than A. labyrinthica. In P. lugubris from both sites, the observed increase in the percentage of metallothionein and Hsp70-positive cells, simultaneous with intensification of superoxide dismutase and catalase activity, suggests an anti-apoptotic function of those proteins in representatives of wandering spiders. In the midgut glands of A. labyrinthica, heat shock and dimethoate increased the number of Annexin V-positive cells as well as the amounts of mitochondria with low transmembrane potential ([DELTA][PSI]m) versus the control. The changes in the percentage of MT and Hsp70-positive cells in funnel web spiders were less than in wolf spiders. The absence of change in SOD and CAT activity in A. labyrinthica shows that the participation of those enzymes in antioxidant reactions is minimal in this species. [copyright] 2005 Elsevier Inc. All rights reserved.

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