TY - JOUR T1 - Apoptosis and biochemical biomarkers of stress in spiders from industrially polluted areas exposed to high temperature and dimethoate JF - Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology Y1 - 2005 A1 - Wilczek, Grazyna SP - 194 EP - 206 KW - [Temperature / High temperature / ] [Poland / Olkusz & Pilica / KW - Abiotic factors KW - Agelena labyrinthica KW - Agelena labyrinthica (Araneae). KW - Apoptosis / Midgut gland KW - Apoptosis & biochemical biomarkers of stress KW - Biochemistry KW - biomarkers KW - Chemical factors KW - Chemical pollution KW - Digestive system KW - enzyme activity / ] [Digestive gland / / Apoptosis & chemical stress KW - Eurasia KW - Europe KW - gland apoptosis & biochemical stress marker responses to temperature & KW - individuals from metal polluted sites] [Enzymes / Antioxidant KW - individuals from polluted sites] [Metal pollution / / Midgut KW - Land zones KW - Life cycle and development KW - metallothionein / Stress biomarkers KW - Midgut gland apoptosis & biochemical stress marker responses] KW - Palaearctic region KW - Pardosa lugubris (Araneae). KW - Pardosa lugubris [Proteins / Heat shock proteins & KW - pesticide KW - pesticide exposure]. KW - pesticide relationship] [Fertilizers and pesticides / Dimethoate / KW - Physical factors KW - Pollution KW - Proteins KW - responses to high temperature & KW - responses to temperature & KW - stress biomarker response to temperature & KW - temperature & pesticide pollution exposure] [Development / AB - The aim of this study was to evaluate the relations between apoptosis and the activity of antioxidant enzymes (superoxide dismutase; catalase) and quantitative changes in stress protein positive cells (Hsp70; metallothionein) in midgut glands of funnel web spiders Agelena labyrinthica (Agelenidae) and wolf spiders Pardosa lugubris (Lycosidae) exposed to high temperature and pesticide under laboratory conditions. The spiders were collected from two meadow ecosystems differently polluted with metals (Olkusz and Pilica, southern Poland). Under stress conditions, P. lugubris had fewer apoptotic cells in the midgut glands than A. labyrinthica. In P. lugubris from both sites, the observed increase in the percentage of metallothionein and Hsp70-positive cells, simultaneous with intensification of superoxide dismutase and catalase activity, suggests an anti-apoptotic function of those proteins in representatives of wandering spiders. In the midgut glands of A. labyrinthica, heat shock and dimethoate increased the number of Annexin V-positive cells as well as the amounts of mitochondria with low transmembrane potential ([DELTA][PSI]m) versus the control. The changes in the percentage of MT and Hsp70-positive cells in funnel web spiders were less than in wolf spiders. The absence of change in SOD and CAT activity in A. labyrinthica shows that the participation of those enzymes in antioxidant reactions is minimal in this species. [copyright] 2005 Elsevier Inc. All rights reserved. VL - 141 SN - 1532-0456 UR - ://ZOOREC:ZOOR14201000233 N1 - ZOOR14201000233 ER -