Agelenids of the World

Systematics and Taxonomy of Agelenidae, a Worldwide distributed Spider Family

Relations between metals (Zn, Pb, Cd and Cu) and glutathione-dependent detoxifying enzymes in spiders from a heavy metal pollution gradient

Publication Type:Journal Article
Year of Publication:2004
Authors:G. Wilczek, Babczynska, A., Augustyniak, M., Migula, P.
Journal:Environmental Pollution
Date Published:2004
ISBN Number:0269-7491
Keywords:/ Relations with glutathione dependent detoxifying enzymes] [Enzymes /, Abiotic factors, Agelena labyrinthica, Agelena labyrinthica (Araneae)., Biochemistry, burden] [Grassland / Meadow / Heavy metal burden & glutathione dependent, Chemical factors, Chemical pollution, detoxifying enzymes] [Metal pollution / / ] [Metals / / ] [Poland /, Eurasia, Europe, Glutathione dependent detoxifying enzymes / Relations with heavy metal, Habitat, Land zones, Olkusz / meadow habitat]., Palaearctic region, Pardosa lugubris (Araneae)., Pardosa lugubris [Pollutants / Heavy metal burdens, Pollution, Proteins, Terrestrial habitat

We studied the relations between glutathione-dependent detoxifying enzymes and heavy metal burdens in the web-building spider Agelena labyrinthica (Agelenidae) and the wolf spider Pardosa lugubris (Lycosidae) from five meadow sites along a heavy metal pollution gradient. We assayed the activity of glutathione-S-transferase (GST) and glutathione peroxidases (GPOX, GSTPx), and glutathione (GSH) levels in both sexes. Except for GSH vs Pb content, we found significant correlations between GPOX and GSTPx activity and metal concentrations in females of A. labyrinthica. The highest activity of these enzymes measured in the web-building spiders was found in the individuals from the most polluted sites. In P. lugubris males significant correlations were found between GST and Pb and Zn concentrations, and between GPOX and GSTPx and the concentration of Cu. GST activity was higher in males collected from less polluted areas. Thus, detoxifying strategies against pollutants seemed to be sex-dependent. Actively hunting spiders had higher metal concentrations, maintaining lower activity of detoxifying enzymes and a lower glutathione level. [copyright] 2004 Elsevier Ltd. All rights reserved.

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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith