Agelenids of the World

Systematics and Taxonomy of Agelenidae, a Worldwide distributed Spider Family

Cellular stress reactions assessed by gender and species in spiders from areas variously polluted with heavy metals

Publication Type:Journal Article
Year of Publication:2008
Authors:G. Wilczek, Babczynska, A., Wilczek, P., Dolezych, B., Migula, P., Mlynska, H.
Journal:Ecotoxicology and Environmental Safety
Date Published:2008
ISBN Number:0147-6513
Keywords:[Cytology / Apoptosis & necrosis / ] [Pollutants / Midgut gland heavy, [Digestive system / / ] [Sex differences / / ] [Grassland / Meadows /, ] [Metal pollution / Heavy metal pollution / ] [Metals / / ] [Poland /, Abiotic, Agelena labyrinthica, Agelena labyrinthica (Araneae)., Biochemistry, Chemical factors, Chemical pollution, Cytology, Digestive system, Eurasia, Europe, factors, Habitat, Land zones, Linyphia triangularis, Linyphia triangularis (Araneae)., metal content / ] [Proteins / Antioxidant parameters / ] [Enzymes / / ], Olkusz & Pilica / ]., Palaearctic region, Pollution, Proteins, Reproduction, Terrestrial habitat, Xerolycosa nemoralis, Xerolycosa nemoralis (Araneae).

In the funnel web spider Agelena labyrinthica (Agelenidae; A. l.), sheet web spider Linyphia triangularis (Linyphiidae; L. t.) and wolf spider Xerolycosa nemoralis (Lycosidae; X. n.) from two differently polluted meadow sites in southern Poland, we studied the relations between antioxidant parameters (glutathione, GSH; glutathione peroxidases, GPOX, GSTPx; catalase, CAT; stress proteins-Hsp70, metallothioneins Mts), the intensity of apoptosis and necrosis, and heavy metal burdens of the midgut gland. Cellular reactions against stress caused by pollutants seemed to be sex-dependent. The concentrations of Zn and Cu in the midgut glands of male A. l. and X. n. were more than double that of the females, from both study sites. In male spiders from the heavily polluted site, both negative correlations (activity of caspase-3-like proteins vs Cu, Zn concentration; number of depolarized mitochondria vs Cu concentration) and positive correlations (number of necrotic cells vs Cu concentrations; activity of CAT vs Zn) were noted. The defense of males against high metal content and its prooxidative effects is based mainly on GSH and CAT. In females the antioxidative reactions are species-specific and depend mainly on high peroxidase activity and on stress protein level. The increase in the number of apoptotic cells in the midgut gland of female spiders from the heavily polluted site suggests the defensive role of this process in maintaining the proper functioning of this organ. (C) 2007 Elsevier Inc. All rights reserved.

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