TY - JOUR T1 - Phylogenetic relationships of Chinese wolf spiders (Lycosidae) inferred from 16S rRNA sequences JF - Acta Arachnologica Sinica Y1 - 2007 A1 - Yan, Yu-Chen A1 - Yan, Heng-Mei SP - 65 EP - 71 KW - / Phylogenetic analysis] [Molecular genetics / / ] [Phylogeny / / KW - Asia KW - Biochemistry KW - Eurasia KW - Evolution KW - Genetics KW - Land zones KW - Lycosidae (Araneae). KW - Lycosidae [Nucleic acids / Mitochondrial 16S rRNA gene partial sequences KW - Palaearctic region KW - partial sequences] [Biochemical variation / / ] [China / / KW - partial sequences]. KW - Phylogenetic relationships inferred from mitochondrial 16S rRNA gene KW - Systematics KW - Taxonomic relationships inferred from mitochondrial 16S rRNA gene KW - Variation AB - The mtDNA- 16S rRNA gene partial sequences (340-360 bp ) were amplified and sequenced from 26 species representing 4 subfamilies and 6 genera of Lycosidae in China. Aligning with 16S rRNA sequences of 2 species(Pardosa Lycosidae) in North America from GenBank, and selecting 1 species Agelena limbata (Agelenidae) as outgroup from China, we constructed a matrix of 29 DNA sequences. Nucleotides composition, transition and transversion, genetic distance of this segment had been analyzed. The phylogenetic trees of the Lycosidae were constructed by using the method of Bayesian and Maximum Parsimony (MP). The results indicate that average A + T content of 16S rRNA gene in Lycosidae is 75 %, which shows a strong A + T bias. The sequences have 157 variable sites and 79 parsimony-informative sites. Genetic distances among genera of Lycosidae are 0.026-0.200. Molecular phylogeny trees suggest that Pirata is a primordial gene of Hippasa which is differentiated earlier from other species. Trochsa is a monophyletic group, it that with Arctosa form a paraphtletic group. The molecular phylogeny of 6 genera of Lycosidae is(Pirata ( Hippasa ( Trochsa+Arctosa( Pardosa + Wadicosa ) ) ) ). VL - 16 SN - 1005-9628 UR - ://ZOOREC:ZOOR14402011486 N1 - ZOOR14402011486 ER -