@article {507, title = {Relations between metals (Zn, Pb, Cd and Cu) and glutathione-dependent detoxifying enzymes in spiders from a heavy metal pollution gradient}, journal = {Environmental Pollution}, volume = {132}, year = {2004}, note = {ZOOR14101004601}, month = {2004}, pages = {453-461}, abstract = {We studied the relations between glutathione-dependent detoxifying enzymes and heavy metal burdens in the web-building spider Agelena labyrinthica (Agelenidae) and the wolf spider Pardosa lugubris (Lycosidae) from five meadow sites along a heavy metal pollution gradient. We assayed the activity of glutathione-S-transferase (GST) and glutathione peroxidases (GPOX, GSTPx), and glutathione (GSH) levels in both sexes. Except for GSH vs Pb content, we found significant correlations between GPOX and GSTPx activity and metal concentrations in females of A. labyrinthica. The highest activity of these enzymes measured in the web-building spiders was found in the individuals from the most polluted sites. In P. lugubris males significant correlations were found between GST and Pb and Zn concentrations, and between GPOX and GSTPx and the concentration of Cu. GST activity was higher in males collected from less polluted areas. Thus, detoxifying strategies against pollutants seemed to be sex-dependent. Actively hunting spiders had higher metal concentrations, maintaining lower activity of detoxifying enzymes and a lower glutathione level. [copyright] 2004 Elsevier Ltd. All rights reserved.}, keywords = {/ Relations with glutathione dependent detoxifying enzymes] [Enzymes /, Abiotic factors, Agelena labyrinthica, Agelena labyrinthica (Araneae)., Biochemistry, burden] [Grassland / Meadow / Heavy metal burden \& glutathione dependent, Chemical factors, Chemical pollution, detoxifying enzymes] [Metal pollution / / ] [Metals / / ] [Poland /, Eurasia, Europe, Glutathione dependent detoxifying enzymes / Relations with heavy metal, Habitat, Land zones, Olkusz / meadow habitat]., Palaearctic region, Pardosa lugubris (Araneae)., Pardosa lugubris [Pollutants / Heavy metal burdens, Pollution, Proteins, Terrestrial habitat}, isbn = {0269-7491}, url = {://ZOOREC:ZOOR14101004601}, author = {Wilczek, Grazyna and Babczynska, Agnieszka and Augustyniak, Maria and Migula, Pawel} } @article {508, title = {Body burdens of metals in spiders from the Lidice coal dump near Ostrava (Czech Republic)}, journal = {Biologia (Bratislava)}, volume = {60}, year = {2005}, note = {ZOOR14205026618}, month = {2005}, pages = {599-605}, abstract = {Spiders{\textquoteright} feeding behaviour and external digestion expose them to man-made pollutants, especially those easily transferred along the food chain. The problem for this study was whether the levels of heavy metals in selected species of spiders from the Lidice coal dump reflect adaptation to environmental pollutants. We used flameless and flame AAS to measure the whole-body concentrations of Cd, Pb, Cu, Zn, Fe, Ni and Mg in male and female spiders differing in their hunting strategies, type of web construction, prey, and taxonomic position (Araneidae, Agelenidae, Linyphiidae, Theridiidae, Tetragnathidae, Lycosidae, Salticidae, Pisauridae, Clubionidae, Philodromidae). The levels of metals found in the spiders were species-dependent, indicating differences related to the hunting strategy and type of prey. Accumulation of Pb, Cu and Zn was always higher in ground spiders than in web-constructing species. Sheet-web spiders Linyphia triangularis and wandering spiders Clubiona lutescens had the lowest Cd, Mg and Cu content of all the studied species. Web-building spiders of the Tetragnathidae family showed the highest Cd, Cu and Pb content, even in species with feeding behaviour similar to spiders of other families. There were no interspecific differences in accumulation only for Fe and Mg. The concentrations of Cd, Ni and Pb were lower in females than in males, irrespective of their taxonomic position and the intensity of their hunting activity. This may suggest that females have better metal-excretion ability than males.}, keywords = {/ Content] [Metals / Heavy metals / ] [Czech Republic / Ostrava, Lidice, / taxonomy \& web type relations]., Abiotic factors, Animal, Araneae (Arachnida)., Araneae [Pollutants / Heavy metal content / foraging strategies, Biochemistry, Chemical factors, Chemical pollution, constructions, Ecology, Eurasia, Europe, Feeding behaviour, Habitat, habitat / Coal dump habitat / ] [Metal pollution / Heavy metal pollution, Land zones, Nutrition, Palaearctic region, Pollution, relations] [Foraging / Hunting strategies / ] [Webs / / Type] [Man made, Systematics, taxonomic position \& web type relations] [Diet / / Heavy metal content}, isbn = {0006-3088}, url = {://ZOOREC:ZOOR14205026618}, author = {Wilczek, Grazyna and Babczynska, Agnieszka and Majkus, Zdenek} } @article {509, title = {Cellular stress reactions assessed by gender and species in spiders from areas variously polluted with heavy metals}, journal = {Ecotoxicology and Environmental Safety}, volume = {70}, year = {2008}, note = {ZOOR14410058564}, month = {2008}, pages = {127-137}, abstract = {In the funnel web spider Agelena labyrinthica (Agelenidae; A. l.), sheet web spider Linyphia triangularis (Linyphiidae; L. t.) and wolf spider Xerolycosa nemoralis (Lycosidae; X. n.) from two differently polluted meadow sites in southern Poland, we studied the relations between antioxidant parameters (glutathione, GSH; glutathione peroxidases, GPOX, GSTPx; catalase, CAT; stress proteins-Hsp70, metallothioneins Mts), the intensity of apoptosis and necrosis, and heavy metal burdens of the midgut gland. Cellular reactions against stress caused by pollutants seemed to be sex-dependent. The concentrations of Zn and Cu in the midgut glands of male A. l. and X. n. were more than double that of the females, from both study sites. In male spiders from the heavily polluted site, both negative correlations (activity of caspase-3-like proteins vs Cu, Zn concentration; number of depolarized mitochondria vs Cu concentration) and positive correlations (number of necrotic cells vs Cu concentrations; activity of CAT vs Zn) were noted. The defense of males against high metal content and its prooxidative effects is based mainly on GSH and CAT. In females the antioxidative reactions are species-specific and depend mainly on high peroxidase activity and on stress protein level. The increase in the number of apoptotic cells in the midgut gland of female spiders from the heavily polluted site suggests the defensive role of this process in maintaining the proper functioning of this organ. (C) 2007 Elsevier Inc. All rights reserved.}, keywords = {[Cytology / Apoptosis \& necrosis / ] [Pollutants / Midgut gland heavy, [Digestive system / / ] [Sex differences / / ] [Grassland / Meadows /, ] [Metal pollution / Heavy metal pollution / ] [Metals / / ] [Poland /, Abiotic, Agelena labyrinthica, Agelena labyrinthica (Araneae)., Biochemistry, Chemical factors, Chemical pollution, Cytology, Digestive system, Eurasia, Europe, factors, Habitat, Land zones, Linyphia triangularis, Linyphia triangularis (Araneae)., metal content / ] [Proteins / Antioxidant parameters / ] [Enzymes / / ], Olkusz \& Pilica / ]., Palaearctic region, Pollution, Proteins, Reproduction, Terrestrial habitat, Xerolycosa nemoralis, Xerolycosa nemoralis (Araneae).}, isbn = {0147-6513}, url = {://ZOOREC:ZOOR14410058564}, author = {Wilczek, Grazyna and Babczynska, Agnieszka and Wilczek, Piotr and Dolezych, Bogdan and Migula, Pawel and Mlynska, Hanna} } @article {30, title = {Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas}, journal = {Ecotoxicology and Environmental Safety}, volume = {74}, year = {2011}, note = {ZOOR14802016758}, month = {2011}, pages = {1498-1503}, abstract = {Spiders inhabiting post industrial environments, such as waste heaps or ore-bearing areas, are exposed to high concentrations of metals, accumulated in the body of their prey and transferred along food chains. Therefore spiders are pressed to develop metal-neutralization strategies. Low-molecular, multifunction proteins: metallothioneins (MTs), often postulated as biomarkers of metal exposure, are known to bind metals and thus protect organisms against their toxic effects. Yet the proteins are still not well recognized in spiders. The aim of this study was to assess, by immunodetection method, ELISA, the concentration of metallothioneins in adult females of three web building spider species: Araneus diadematus (Araneidae), Agelena labyrinthica (Agelenidae) and Linyphia triangularis (Linyphiidae) from three variously polluted areas in southern Poland: Olkusz, ore-bearing post industrial site; Katowice[long dash]WeLnowiec: post metallurgic waste heap, Pilica: the reference, rural, area. The concentration of metallothioneins has been analyzed in relation to the metal concentration in spiders body. The study gives the evidence that metallothioneins are reliably detectable by means of ELISA technique. The analysis of results obtained shows a strong species-dependence of the MTs level. Positive correlations between MTs concentration and metal body burden (mainly Zn and Pb) were found. This suggests that the proteins play an important role in the neutralization and regulation of metal ions in spiders. The same correlation indicate the possibility to consider MTs in spiders as biomarkers of metal exposure and effects. However, the species specificity as well as metal characteristics should be taken under account.}, keywords = {Agelena labyrinthica, Agelena labyrinthica (Araneae)., Araneus diadematus, Araneus diadematus (Araneae)., Biochemical techniques, Biochemistry, Chemical, Eurasia, Europe, Land zones, Linyphia triangularis (Araneae)., Linyphia triangularis [Assay, Metallothioneins content / ] [Metal pollution / / ] [Poland / South /, Palaearctic region, Pollution, Techniques, techniques / ELISA / ] [Pollutants / Metals content / ] [Proteins /}, isbn = {0147-6513}, url = {://ZOOREC:ZOOR14802016758}, author = {Babczynska, Agnieszka and Wilczek, Grazyna and Szulinska, Elzbieta and Franiel, Izabella} }